How to Reconstitute Peptides: Step-by-Step

What Do I Need to Reconstitute a Peptide?

• The peptide vial
• Bacteriostatic water (sterile water with a preservative)
• New sterile syringes and needles
• Alcohol swabs
• A sharps container

How Do I Reconstitute a Peptide, Step by Step?

1. Prepare. Wash your hands and clean a flat surface. Let the vial reach room temperature.
2. Clean the stoppers. Wipe the top of the peptide vial and the water vial with an alcohol swab and let them dry.
3. Draw the water. Draw the amount of bacteriostatic water you planned to add. See the math below.
4. Add it slowly. Insert the needle into the peptide vial and let the water run down the inside wall of the glass. Do not spray it onto the powder.
5. Dissolve gently. Swirl the vial slowly or let it sit. Never shake it, since shaking can damage the peptide.
6. Check it. The liquid should be clear. If it is cloudy or has particles, do not use it. See the appearance guide.
7. Label and store. Write the date and the concentration on the vial and keep it refrigerated and away from light.

How Do I Calculate the Concentration?

Concentration = mg of peptide ÷ mL of water added. A 5 mg vial with 2 mL of water is 2.5 mg per mL. On a 100-unit (1 mL) insulin syringe, 1 unit equals 0.01 mL, so 10 units is 0.1 mL. Always check your numbers twice. See the measurement guide.

Educational information only. This is general information, not medical advice, and no peptide covered here is approved by Health Canada for human use. Talk to a licensed healthcare professional about your health.

Frequently Asked Questions